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A simple fragile X PCR assay with 7-deazaguanine-substituted DNA visualized by ethidium bromide.

Molecular and cellular probes - 1 Apr 1994

Cao J, Tarleton J, Barberio D, Davidow L S

Abstract excerpt

A deazaguanine-substituted DNA PCR product from FMR-1 (the fragile X mental retardation syndrome gene) can be efficiently visualized with ethidium bromide on standard agarose gels. Normal-sized alleles (less than 54 CGG repeats) generated strong, easily visible bands in the expected size range of 491-635 bp. Southern blot analysis and radioactive PCR on sequencing gels were used to verify that the 74 males (out...

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PMID: 7935515
DOI: 10.1006/mcpr.1994.1023

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