Article
A simple fragile X PCR assay with 7-deazaguanine-substituted DNA visualized by ethidium bromide.
Molecular and cellular probes - 1 Apr 1994
Cao J, Tarleton J, Barberio D, Davidow L S
Abstract excerpt
A deazaguanine-substituted DNA PCR product from FMR-1 (the fragile X mental retardation syndrome gene) can be efficiently visualized with ethidium bromide on standard agarose gels. Normal-sized alleles (less than 54 CGG repeats) generated strong, easily visible bands in the expected size range of...
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- DNA
- Ethidium
- Fragile X Syndrome
- Guanine
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction