Article
Oligonucleotide fingerprinting with (CAC)5: nonradioactive in-gel hybridization and isolation of individual hypervariable loci.
Electrophoresis - 1 Jan 2000
Zischler H, Hinkkanen A, Studer R
Abstract excerpt
The first topic to be treated in this paper is the nonradioactive DNA fingerprinting by means of in-gel hybridization with digoxigenated (CAC)5. Besides the fact that time-consuming Southern blotting can be avoided, the dried agarose is an excellent matrix to produce background-free nonradioactive DNA fingerprints. There is no tendency of either the oligonucleotide probe or the antibody towards unspecific binding...
Topics
- Base Sequence
- DNA
- DNA Fingerprinting
- DNA Probes
- Genetic Variation
- Genomic Library
- Humans
- Male
- Molecular Sequence Data
- Placenta
- Polymerase Chain Reaction