Article
The diversity of antigen-specific monoclonal antibodies from transgenic mice bearing human immunoglobulin gene miniloci.
European journal of immunology - 1 Nov 1994
Wagner S D, Popov A V, Davies S L, Xian J, Neuberger M S, Brüggemann M
Abstract excerpt
An approach to the preparation of antigen-specific human monoclonal antibodies focuses on mice transgenic for human immunoglobulin gene miniloci; the V gene segments in these miniloci undergo productive rearrangement to yield mouse B cells expressing human immunoglobulin (Ig) chains. The general usefulness of this strategy hinges on whether it is feasible to obtain specific, high-affinity antibodies following...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Affinity
- Antibody Diversity
- Base Sequence
- Genes, Immunoglobulin
- Humans
- Immunization
- Immunoglobulin Heavy Chains
- Mice