Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts.

doi:10.1002/em.2850240304

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Article

Induction of DNA strand breaks by ethylene oxide in human diploid fibroblasts.

Environmental and molecular mutagenesis - 1 Jan 1994

Nygren J, Cedervall B, Eriksson S, Dusinská M, Kolman A

Abstract excerpt

In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to meas...

Topics

Cells, Cultured
Colony-Forming Units Assay
DNA
DNA Damage
DNA, Single-Stranded
Diploidy
Dose-Response Relationship, Drug
Electrophoresis, Gel, Pulsed-Field
Ethylene Oxide
Fibroblasts
Gamma Rays
Humans
Mutation
Temperature

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