Article
Translational repression by bacteriophage MS2 coat protein expressed from a plasmid. A system for genetic analysis of a protein-RNA interaction.
The Journal of biological chemistry - 5 Apr 1990
Peabody D S
Abstract excerpt
The coat protein of bacteriophage MS2 is a translational repressor. It inhibits the synthesis of the viral replicase by binding a specific RNA structure that contains the replicase translation initiation region. In order to begin a genetic dissection of the repressor activity of coat protein, a t...
Topics
- Bacteriophages
- Base Sequence
- Blotting, Western
- Capsid
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Biosynthesis
- RNA, Viral
- Recombinant Fusion Proteins
- Recombinant Proteins
- Repressor Proteins
- Transcription Factors
- Transformation, Bacterial
- beta-Galactosidase