Article
Amino acid replacements in the Serratia marcescens haemolysin ShIA define sites involved in activation and secretion.
Molecular microbiology - 1 Sept 1993
Schönherr R, Tsolis R, Focareta T, Braun V
Abstract excerpt
The haemolysin of Serratia marcescens (ShIA) is translocated through the cytoplasmic membrane by the signal peptide-dependent export apparatus. Translocation across the outer membrane (secretion) is mediated by the ShIB protein. Only the secreted form of ShIA is haemolytic. ShIB also converts in...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genetic Complementation Test
- Genotype
- Hemolysin Proteins
- Hemolysis
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Peptide Fragments
- Plasmids
- Point Mutation
- Serratia marcescens
- Trypsin