Article
A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae.
Molecular microbiology - 1 Dec 1993
Larsson K, Ansell R, Eriksson P, Adler L
Abstract excerpt
Osmoregulatory mutants of Saccharomyces cerevisiae with a defect in their capacity to readjust the cell volume/buoyant density after osmotically induced dehydration were enriched by density gradient centrifugation. Colonies derived from cells that remained dense after dehydration were screened for sensitivity to high concentrations of NaCl and defects in their osmotically induced production and intracellular...
Topics
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Genes, Fungal
- Genetic Complementation Test
- Glycerol-3-Phosphate Dehydrogenase (NAD+)
- Glycerolphosphate Dehydrogenase
- Molecular Sequence Data
- Mutation
- Phenotype