Article
Osmoregulation of fission yeast: cloning of two distinct genes encoding glycerol-3-phosphate dehydrogenase, one of which is responsible for osmotolerance for growth.
Molecular microbiology - 1 Dec 1995
Ohmiya R, Yamada H, Nakashima K, Aiba H, Mizuno T
Abstract excerpt
Many types of microorganisms, including both prokaryotes and eukaryotes, have developed mechanisms to adapt to severe osmotic stress. In this study, we isolated multicopy suppressor genes for a Schizosaccharomyces pombe mutant, which exhibited the clear phenotype of being osmosensitive for growth (Osms) on agar plates containing high concentrations of either non-ionic or ionic osmotic solutes. Two genes were thus...
Topics
- Adaptation, Physiological
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genetic Complementation Test
- Glycerol
- Glycerolphosphate Dehydrogenase
- Molecular Sequence Data
- Osmolar Concentration
- Phenotype