Article
Cloning, sequencing, and expression of RecA proteins from three distantly related thermophilic eubacteria.
The Journal of biological chemistry - 14 Oct 1994
Wetmur J G, Wong D M, Ortiz B, Tong J, Reichert F, Gelfand D H
Abstract excerpt
Sequences of the recA genes of the highly divergent thermopholic eubacteria Thermus aquaticus (and Thermus thermophilus), Thermotoga maritima, and Aquifex pyrophilus were determined from fragments derived by polymerase chain reaction (PCR) with degenerate primers and from inverse PCR products obt...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- Genes, Bacterial
- Genetic Variation
- Gram-Negative Aerobic Bacteria
- Gram-Negative Anaerobic Bacteria
- Hot Temperature
- Hydrolysis
- Isoelectric Point
- Molecular Sequence Data
- Protein Denaturation
- Rec A Recombinases
- Recombinant Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid