Article
Alteration of the pilin adhesin of Pseudomonas aeruginosa PAO results in normal pilus biogenesis but a loss of adherence to human pneumocyte cells and decreased virulence in mice.
Infection and immunity - 1 Oct 1994
Farinha M A, Conway B D, Glasier L M, Ellert N W, Irvin R T, Sherburne R, Paranchych W
Abstract excerpt
The disulfide loop domain of Pseudomonas aeruginosa PAO pilin was altered by insertion of a chloramphenicol acetyltransferase gene into the pilin gene so that the C-terminal nine amino acids were replaced with 11 new amino acids. The altered pilin gene was transferred into wild-type PAO by recomb...
Topics
- Adhesins, Bacterial
- Amino Acid Sequence
- Animals
- Bacterial Adhesion
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Female
- Fimbriae Proteins
- Fimbriae, Bacterial
- Humans
- Lectins
- Lung
- Male