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Article

The position of the target site for engineered nucleases improves the aberrant mRNA clearance in in vivo genome editing.

Scientific reports - 6 Mar 2020

Lee Jae Hoon, Yu Sungsook, Nam Tae Wook, Roh Jae-Il, Jin Young, Han Jeong Pil, Cha Ji-Young, Kim Yoon Ki, Yeom Su-Cheong, Nam Ki Taek, Lee Han-Woong

Abstract excerpt

Engineered nucleases are widely used for creating frameshift or nonsense mutations in the target genes to eliminate gene functions. The resulting mRNAs carrying premature termination codons can be eliminated by nonsense-mediated mRNA decay. However, it is unclear how effective this process would be in vivo. Here, we found that the nonsense-mediated decay was unable to remove the mutant mRNAs in twelve out of...

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Identifiers

PMID: 32144373
DOI: 10.1038/s41598-020-61154-4
PMCID: PMC7060192

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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