Article
Genomic fingerprinting of Yersinia enterocolitica species by degenerate oligonucleotide-primed polymerase chain reaction.
Electrophoresis - 1 May 1994
Sayada C, Picard B, Elion J, Krishnamoorthy R
Abstract excerpt
A novel version of the polymerase chain reaction (PCR) termed degenerate oligonucleotide-primed PCR (DOP-PCR) was used to fingerprint the bacterial genome of Yersinia enterocolitica species. Eight well-characterized reference strains of Y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. Optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a...
Topics
- Base Sequence
- DNA Fingerprinting
- DNA Primers
- Genome, Bacterial
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Yersinia enterocolitica