Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand.

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Article

Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand.

The Southeast Asian journal of tropical medicine and public health - 1 Mar 2007

Pannanusorn Srisuda, Chaiprasert Angkana, Prariyachatigul Chularut, Krajaejun Theerapong, Vanittanakom Nongnuch, Chindamporn Ariya, Wanachiwanawin Wanchai, Satapatayavong Boonmee

Abstract excerpt

Forty-three Pythium insidiosum clinical isolates recovered from human pythiosis cases in Thailand were characterized by random amplified polymorphic DNA (RAPD) analysis. Three random oligonucleotide primers, OPW11, OPW12 and OPX13 generated 39, 34 and 35 DNA patterns with high value of typeability (100%), reproducibility (98.5, 88.8 and 93.3%) and discriminatory power (0.83, 0.82 and 0.77), respectively. Using...

Topics

DNA Fingerprinting
DNA Primers
Genotype
Humans
Infections
Phylogeny
Polymorphism, Genetic
Pythium
Random Amplified Polymorphic DNA Technique
Thailand
Virulence

Source

Open in PubMed

Identifiers

PMID: 17539291

Provenance

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