Multiplex primer-extension assay for identification of Yersinia species.

doi:10.1007/s11033-014-3555-1

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Article

Multiplex primer-extension assay for identification of Yersinia species.

Molecular biology reports - 1 Oct 2014

Dalmasso Alessandra, Civera Tiziana, Filipello Virginia, Bottero Maria Teresa

Abstract excerpt

A multiplex primer-extension reaction (PER) assay, was specifically designed for the identification of ten Yersinia species. The assay, directed towards the tufA (elongation factor Tu) gene, was tested on a total of 42 samples representing Yersinia species and non-Yersinia species. The primers used in the preliminary PCR, designed in highly conserved regions upstream and downstream of the diagnosis sites,...

Topics

Evolution, Molecular
Genes, Bacterial
Genetic Variation
Molecular Typing
Yersinia

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Open in PubMed

Identifiers

PMID: 24985982
DOI: 10.1007/s11033-014-3555-1

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