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Article

Multiplex primer-extension assay for identification of Yersinia species.

Molecular biology reports - 1 Oct 2014

Dalmasso Alessandra, Civera Tiziana, Filipello Virginia, Bottero Maria Teresa

Abstract excerpt

A multiplex primer-extension reaction (PER) assay, was specifically designed for the identification of ten Yersinia species. The assay, directed towards the tufA (elongation factor Tu) gene, was tested on a total of 42 samples representing Yersinia species and non-Yersinia species. The primers used in the preliminary PCR, designed in highly conserved regions upstream and downstream of the diagnosis sites,...

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PMID: 24985982
DOI: 10.1007/s11033-014-3555-1

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