Amplification of anonymous DNA fragments using pairs of long primers generates reproducible DNA fingerprints that are sensitive to genetic variation.

doi:10.1002/elps.1150180904

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Article

Amplification of anonymous DNA fragments using pairs of long primers generates reproducible DNA fingerprints that are sensitive to genetic variation.

Electrophoresis - 1 Aug 1997

Gillings M, Holley M

Abstract excerpt

The reproducibility and potential applications of anonymous amplification protocols can be improved by using pairs of primers, each of 18 to 24 bases, to replace the single 8 to 10 base primers normally used in randomly amplified polymorphic DNA (RAPD) or DNA amplification fingerprinting (DAF) me...

Topics

Animals
DNA
DNA Fingerprinting
DNA Primers
DNA, Bacterial
DNA, Fungal
DNA, Viral
Gene Amplification
Genetic Variation
Minisatellite Repeats
Random Amplified Polymorphic DNA Technique
Reproducibility of Results

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