Article
Functional consequences of glycine mutations in the predicted cytoplasmic loops of P-glycoprotein.
The Journal of biological chemistry - 11 Mar 1994
Loo T W, Clarke D M
Abstract excerpt
Site-directed mutagenesis was used to investigate whether glycine residues in the predicted cytoplasmic loops play essential roles in the structure and function of human P-glycoprotein. Mutant cDNAs in which codons for each of the 20 glycine residues were changed to valine, were expressed in mous...
Topics
- 3T3 Cells
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Affinity Labels
- Amino Acid Sequence
- Animals
- Azides
- Carrier Proteins
- Cloning, Molecular
- DNA, Complementary
- Dihydropyridines
- Drug Resistance
- Glycine
- Glycoside Hydrolases
- Hot Temperature
- Membrane Glycoproteins
- Mice
- Molecular Sequence Data
- Mutation