Prolonged association of temperature-sensitive mutants of human P-glycoprotein with calnexin during biogenesis.

Abstract excerpt

Mutation of amino acids located within or immediately NH2-terminal to transmembrane segment 7 of human P-glycoprotein abolished the ability of the protein to confer resistance to cytotoxic drugs. Each of these mutant P-glycoproteins had an apparent mass of 150 kDa, compared with 170 kDa for wild-type P-glycoprotein, and the apparent mass was altered by endoglycosidase H digestion. These observations suggest that...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to 3T3 Cells
• Share to Amino Acid Sequence
• Share to Kinetics