Article
Phoenix mutagenesis: one-step reassembly of multiply cleaved plasmids with mixtures of mutant and wild-type fragments.
Analytical biochemistry - 1 Nov 1993
Berger S L, Manrow R E, Lee H Y
Abstract excerpt
A method for site-specific mutagenesis which can be used with any gene in any vector has been devised. This method has been named "phoenix mutagenesis." Upon selection of the mutation site, the plasmid bearing the gene in question is cleaved with one or two restriction enzymes that generate fragments with random staggered ends. The enzymes are chosen to maximize the protrusions while minimizing the size of the...
Topics
- Base Sequence
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Protein Precursors
- Reference Values
- Thymosin