Molecular mechanism of potentially lethal damage repair. I. Enhanced fidelity of DNA double-strand break rejoining under conditions allowing potentially lethal damage repair.

Abstract excerpt

This study contributes to the elucidation of the molecular mechanism underlying potentially lethal damage (PLD) repair. Repair of DNA double-strand breaks (dsbs) is involved in PLD repair in yeast, i.e. in the enhanced survival of cells due to post-irradiation treatment under non-growth conditions before plating cells on nutrient agar (growth conditions). However, dsbs are rejoined when cells are kept either in...

Topics

• DNA Repair
• DNA, Fungal
• Electrophoresis, Gel, Pulsed-Field
• Escherichia coli
• Mutation
• Saccharomyces cerevisiae
• Transformation, Genetic