Article
Genetic analysis of human DNA recovered from minute amounts of serum or plasma.
Journal of immunological methods - 13 Mar 1995
Fowke K R, Plummer F A, Simonsen J N
Abstract excerpt
A rapid and inexpensive method is described where a small amount of serum or plasma was used as the source of DNA for genetic analysis. Using a silica gel matrix DNA was isolated from 50 microliters of archived serum or plasma. The specimens were collected from 13 individuals at two separate time points 3-6 years apart. The polymorphic region of second exon of the MHC class II gene HLA DQA1 was amplified using...
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