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Article

Direct PCR of small genomic DNA fragments from serum.

BioTechniques - 1 Nov 1997

Sandford A J, Paré P D

Abstract excerpt

A simple and rapid method is described where human genomic DNA suitable for PCR was prepared from serum by microwave irradiation. We were able to reproducibly amplify single-copy gene sequences up to 442 bp from small quantities of serum without the need for DNA extraction. Genotyping results obtained from serum samples were shown to be identical to those derived from purified DNA from the same individuals.

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Source

Identifiers

PMID: 9383555
DOI: 10.2144/97235st05

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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