Article
Malignant progression of B16 melanoma cells induced in vitro by growth factors produced by highly malignant cells.
Clinical & experimental metastasis - 1 Mar 1995
Stackpole C W, Groszek L, Kalbag S S
Abstract excerpt
Four mouse B16 melanoma subclones (G3.15, G3.5, G3.12 and G3.26) exhibit progressively greater growth capacity in vitro and in vivo. Previously, non-metastatic G3.15 cells were sequentially converted, in monolayer cultures, to the moderately-metastatic G3.5 cells, and then to a highly-metastatic G3.5* phenotype. Both conversions were induced by hypoxia followed by confluence, and also occurred in tumors. G3.5*...
Topics
- Animals
- Cell Division
- Culture Media, Serum-Free
- Female
- Growth Substances
- Hydrogen-Ion Concentration
- Melanoma, Experimental
- Mice
- Mice, Inbred C57BL
- Phenotype
- Tumor Cells, Cultured