Article
Clustering of multiple transgene integrations in highly-unstable Ascobolus immersus transformants.
Current genetics - 1 Oct 1994
Rhounim L, Grégoire A, Salama S, Faugeron G
Abstract excerpt
A large proportion of Ascobolus immersus transformants are highly unstable in crosses: the phenotype conferred by the transgene is not transmitted to the progeny, irrespective of the endogenous or foreign origin of the transgene. They all have integrated multiple transgene copies, clustered at a single chromosomal site or at tightly-linked sites. Clustered non-homologous integrations are always rearranged. Yet...
Topics
- Ascomycota
- Crosses, Genetic
- DNA, Fungal
- Genes, Fungal
- Multigene Family
- Phenotype
- Plasmids
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping
- Transformation, Genetic