Article
Detection of Clostridium botulinum type F using the polymerase chain reaction.
Molecular and cellular probes - 1 Oct 1994
Ferreira J L, Hamdy M K, McCay S G, Hemphill M, Kirma N, Baumstark B R
Abstract excerpt
The polymerase chain reaction (PCR) was used to amplify a portion of the Clostridium botulinum type F toxin gene. An 1137-bp fragment was amplified from 11 different strains of type F C. botulinum with primers derived from the published sequence of type F strain no. 202. This fragment was not amplified from the DNA of C. botulinum types A, B and E, or from other clostridial organisms examined. When used as a...
Topics
- Animals
- Base Sequence
- Botulinum Toxins
- Clostridium botulinum
- DNA Primers
- DNA Probes
- DNA, Bacterial
- Genes, Bacterial
- Genetic Variation
- Mice
- Molecular Sequence Data