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Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction

1 Sept 1993

Abstract excerpt

The polymerase chain reaction (PCR) was used as the basis for the development of highly sensitive and specific diagnostic tests for organisms harboring botulinum neurotoxin type A through E genes. Synthetic DNA primers were selected from nucleic acid sequence data for Clostridium botulinum neurotoxins. Individual components of the PCR for each serotype (serotypes A through E) were adjusted for optimal...

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PMID: 8215372
DOI: 10.1128/aem.59.9.3011-3020.1993

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