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Multiplex PCR Assay for Detection and Identification of<i>Clostridium botulinum</i>Types A, B, E, and F in Food and Fecal Material

1 Dec 2001

Abstract excerpt

Botulism is diagnosed by detecting botulinum neurotoxin and Clostridium botulinum cells in the patient and in suspected food samples. In this study, a multiplex PCR assay for the detection of Clostridium botulinum types A, B, E, and F in food and fecal material was developed. The method employs four new primer pairs with equal melting temperatures, each being specific to botulinum neurotoxin gene type A, B, E, or...

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Identifiers

PMID: 11722924
DOI: 10.1128/aem.67.12.5694-5699.2001

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