Article
Soj (ParA) DNA binding is mediated by conserved arginines and is essential for plasmid segregation.
Proceedings of the National Academy of Sciences of the United States of America - 18 Dec 2007
Hester Christina M, Lutkenhaus Joe
Abstract excerpt
Soj is a member of the ParA family of ATPases involved in plasmid and chromosomal segregation. It binds nonspecifically and cooperatively to DNA although the function of this binding is unknown. Here, we show that mutation of conserved arginine residues that map to the surface of Bacillus subtilis Soj caused only minimal effects on nucleotide-dependent dimerization but had dramatic effects on DNA binding. Using a...
Topics
- Amino Acid Sequence
- Arginine
- Bacillus subtilis
- Bacterial Proteins
- Cell Nucleus
- Chromosome Segregation
- Chromosomes, Bacterial
- Conserved Sequence
- DNA, Bacterial
- Dimerization
- F Factor
- Green Fluorescent Proteins
- Molecular Sequence Data
- Mutation