Article
Disruption of the F plasmid partition complex in vivo by partition protein SopA.
Molecular microbiology - 1 Nov 2000
Lemonnier M, Bouet J Y, Libante V, Lane D
Abstract excerpt
The SopA protein plays an essential, though so far undefined, role in partition of the mini-F plasmid but, when overproduced, it causes loss of mini-F from growing cells. Our investigation of this phenomenon has revealed that excess SopA protein reduces the linking number of mini-F. It appears to do so by disturbing the partition complex, in which SopB normally introduces local positive supercoiling upon binding...
Topics
- Bacterial Proteins
- Binding Sites
- Culture Media
- Escherichia coli
- Escherichia coli Proteins
- F Factor
- Phenotype