[Application of PCR and RT-PCR method to molecular biology study in nephrology].

Abstract excerpt

Recent advance in molecular biology and genetic engineering has made a profound influence on basic and clinical medicine. Development of PCR (polymerase chain reaction) has transformed our trend of thought in molecular biology. Applications of PCR method has extended to nephrology study. RT (reverse transcription)-PCR method was elaborated to identify mRNA even in a small number of cells. It is also important and...

Topics

• Cloning, Molecular
• DNA
• Humans
• Mutation
• Nephrology
• Nucleic Acid Hybridization
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Restriction Fragment Length
• RNA-Directed DNA Polymerase