Article
In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis.
Infection and immunity - 1 Mar 1995
McAdam R A, Weisbrod T R, Martin J, Scuderi J D, Brown A M, Cirillo J D, Bloom B R, Jacobs W R
Abstract excerpt
Insertional mutagenesis in Mycobacterium bovis BCG, a member of the slow-growing M. tuberculosis complex, was accomplished with transposons engineered from the Mycobacterium smegmatis insertion element IS1096. Transposons were created by placing a kanamycin resistance gene in several different positions in IS1096, and the resulting transposons were electroporated into BCG on nonreplicating plasmids. These...
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