Article
In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis.
Infection and immunity - 1 Mar 1995
McAdam R A, Weisbrod T R, Martin J, Scuderi J D, Brown A M, Cirillo J D, Bloom B R, Jacobs W R
Abstract excerpt
Insertional mutagenesis in Mycobacterium bovis BCG, a member of the slow-growing M. tuberculosis complex, was accomplished with transposons engineered from the Mycobacterium smegmatis insertion element IS1096. Transposons were created by placing a kanamycin resistance gene in several different po...
Topics
- Animals
- Base Sequence
- DNA Transposable Elements
- Genes, Bacterial
- Genetic Complementation Test
- Leucine
- Lung
- Male
- Methionine
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Mycobacterium bovis