Article
Phenotypic analysis of yellow fever virus derived from complementary DNA.
The American journal of tropical medicine and hygiene - 1 Jan 1995
Marchevsky R S, Mariano J, Ferreira V S, Almeida E, Cerqueira M J, Carvalho R, Pissurno J W, da Rosa A P, Simoes M C, Santos C N
Abstract excerpt
A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin-mediated RNA transfection of certified primary cultures of chicken embryo...
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