Gene expression in mycobacteria: transcriptional fusions based on <i>xylE</i> and analysis of the promoter region of the response regulator <i>mtrA</i> from <i>Mycobacterium tuberculosis</i>

Abstract excerpt

Understanding promoter regulation and signal-transduction systems in pathogenic mycobacteria is critical for uncovering the processes that govern interactions of these bacteria with the human host. In order to develop additional genetic tools for analysis of mycobacterial promoters, the xyIE gene from Pseudomonas was tested as a transcriptional fusion reporter in fast- and slow-growing mycobacteria. Initially,...

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