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Article

Promoter analysis in mycobacteria using xyIE reporter assays and its implication in high throughput screening.

Tuberculosis (Edinburgh, Scotland) - 1 Jan 2001

Dastur A, Varshney U

Abstract excerpt

Understanding the molecular biology of Mycobacterium tuberculosis has become the focus of recent studies in infectious diseases. Here, we describe a spectrophotometric method for quantitative analysis of promoters in mycobacteria. The assay is a modification of the xyIE reporter assay, which uses an inexpensive substrate, catechol. The distinguishing feature of this protocol is that cells are grown by patching on...

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Identifiers

PMID: 11584594
DOI: 10.1054/tube.2001.0298

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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