Article
Protein fragments as models for events in protein folding pathways: protein engineering analysis of the association of two complementary fragments of the barley chymotrypsin inhibitor 2 (CI-2).
Biochemistry - 7 Feb 1995
Ruiz-Sanz J, de Prat Gay G, Otzen D E, Fersht A R
Abstract excerpt
Two fragments of chymotrypsin inhibitor-2, CI-2(20-59) and CI-2(60-83), derived from cyanogen bromide cleavage at Met-59, associate to give a native-like structure. We analyze the kinetics and equilibria of association of mutant fragments derived from cleaving mutant proteins at the same methioni...
Topics
- Circular Dichroism
- Energy Metabolism
- Kinetics
- Mutation
- Peptide Fragments
- Peptides
- Plant Proteins
- Protein Denaturation
- Protein Engineering
- Protein Folding
- Protein Structure, Secondary