Article
A simple method for assessing the mutational effect on the protein-DNA interaction: application to amino acid substitutions.
Protein engineering - 1 Sept 1994
Wang Y, Sarai A
Abstract excerpt
We have developed a simple method to assess the effect of mutations on the binding affinity change in the protein-DNA interaction. In this method, we evaluate the contribution of the local geometric effect in protein-DNA recognition by calculating the change in interaction energy caused by mutati...
Topics
- Amino Acid Sequence
- Binding Sites
- DNA
- DNA-Binding Proteins
- In Vitro Techniques
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Engineering
- Proteins
- Repressor Proteins
- Thermodynamics