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Article

Site-directed mutagenesis of cysteine-195 in isocitrate lyase from Escherichia coli ML308.

The Biochemical journal - 1 Jan 1995

Robertson A G, Nimmo H G

Abstract excerpt

Cysteine-195 was previously identified as a probable active site residue in isocitrate lyase (ICL) from Escherichia coli ML308 [Nimmo, Douglas, Kleanthous, Campbell and MacKintosh (1989) Biochem. J. 261, 431-435]. This residue was replaced with serine and alanine residues by site-directed mutagenesis. The mutated genes expressed proteins with low but finite ICL activity, which co-migrated with wild-type ICL on...

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PMID: 7826335
DOI: 10.1042/bj3050239
PMCID: PMC1136455

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