Site-directed mutagenesis of cysteine-195 in isocitrate lyase from Escherichia coli ML308.

doi:10.1042/bj3050239

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Article

Site-directed mutagenesis of cysteine-195 in isocitrate lyase from Escherichia coli ML308.

The Biochemical journal - 1 Jan 1995

Robertson A G, Nimmo H G

Abstract excerpt

Cysteine-195 was previously identified as a probable active site residue in isocitrate lyase (ICL) from Escherichia coli ML308 [Nimmo, Douglas, Kleanthous, Campbell and MacKintosh (1989) Biochem. J. 261, 431-435]. This residue was replaced with serine and alanine residues by site-directed mutagen...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
Cysteine
Escherichia coli
Gene Expression
Hydrogen-Ion Concentration
Isocitrate Lyase
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation

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