Article
Modification of glutathione S-transferase 3-3 mutants with 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone. Identification of the C-terminal tryptic fragment as part of the H-site and evidence that 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone is not specific for cysteine labelling.
The Biochemical journal - 15 Dec 1994
Hong J L, Liu L F, Wang L Y, Tsai S P, Hsieh C H, Hsiao C D, Tam M F
Abstract excerpt
A triple mutant of rat liver glutathione S-transferase 3-3 that has all three cysteine residues replaced with serine (CallS) and a quadruple mutant with a Tyr-115 to phenylalanine substitution on CallS (CallSY115F) were reacted with 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone (GS-1,4-TCBQ...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Chloranil
- Cysteine
- Dithiothreitol
- Enzyme Inhibitors
- Glutathione
- Glutathione Transferase
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- Peptide Fragments