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Article

Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Motohashi Ken, Romano Patrick G N, Hisabori Toru

Abstract excerpt

Thioredoxins (Trx) are a ubiquitous family of proteins that modulate the enzymatic activity of their substrate proteins by redox regulation. This is achieved by reduction of a disulfide bond within their target proteins. A conserved pair of cysteine residues in Trx is required for catalysis of the dithiol-disulfide exchange with their target proteins. A single-cysteine mutant capable of forming a stable mixed...

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PMID: 19083171
DOI: 10.1007/978-1-59745-289-2_8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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