Article
Mapping the mouse ZP3 combining site for sperm by exon swapping and site-directed mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America - 3 Jan 1995
Kinloch R A, Sakai Y, Wassarman P M
Abstract excerpt
During fertilization in mice, sperm bind to mouse ZP3 (mZP3), a M(r) approximately 83,000 glycoprotein present in the ovulated egg extracellular coat, or zona pellucida. Sperm recognize and bind to specific serine/threonine-linked (O-linked) oligosaccharides present at the mZP3 combining site for sperm. Binding to mZP3 induces sperm to undergo a form of exocytosis, the acrosome reaction. To map the mZP3 combining...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Biological Evolution
- Blotting, Northern
- Blotting, Western
- Cell Adhesion
- Cell Line
- DNA Primers
- Egg Proteins