Article
Deletion of lysine 121 creates a temperature-sensitive alteration in insulin binding by the insulin receptor.
The Journal of biological chemistry - 6 Jan 1995
Liu R, Zhu J, Jospe N, Furlanetto R W, Bastian W, Livingston J N
Abstract excerpt
Recently we reported the deletion of Lys-121 in one allele of the insulin receptor gene from a child with severe insulin resistance. In the present work, this mutant receptor (M121) was shown to have an abnormal sensitivity to temperature and an alteration in "negative cooperativity." In contrast to the wild-type receptor (HIRC), insulin binding by the M121 receptor was rapidly and irreversibly lost at...
Topics
- Alkylating Agents
- Animals
- Binding Sites
- CHO Cells
- Cell Membrane
- Cricetinae
- Hot Temperature
- Humans
- Insulin
- Lysine
- Mutation
- Oxidation-Reduction