Article
Expression of the myotonin protein kinase gene in preimplantation human embryos.
Human molecular genetics - 1 Mar 1995
Daniels R, Kinis T, Serhal P, Monk M
Abstract excerpt
We have examined the expression of the myotonin protein kinase gene (MPK) in individual human preimplantation embryos. An allele-specific, reverse transcriptase polymerase chain reaction assay was developed to the required sensitivity using human buccal cells. Human cleavage stage embryos, derived by in vitro fertilisation, were assayed for MPK transcription directly, or after biopsy of a single blastomere used...
Topics
- Alleles
- Blastocyst
- Female
- Gene Expression Regulation, Developmental
- Genotype
- Humans
- Male
- Myotonin-Protein Kinase
- Ovum
- Polymerase Chain Reaction
- Protein Kinases
- Protein Serine-Threonine Kinases