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Extracellular embryo genomic DNA and its potential for genotyping applications

27 Jul 2015

Abstract excerpt

Background: Preimplantation genetic diagnosis (PGD) currently relies on biopsy of one or few embryo cells. Our aim was to evaluate the embryo extracellular matrices (spent medium and blastocoele fluid) as source of DNA for embryo genotyping. Results/methodology: We first evaluated the amplifiability and the amount of genomic DNA in spent embryo culture media from day 3 (n = 32) and day 5/6 (n = 54). Secondly, we...

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PMID: 28031914
DOI: 10.4155/fso.15.62

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