Article
A site-directed mutant of Cu,Zn-superoxide dismutase modeled after native extracellular superoxide dismutase.
Biological trace element research - 1 Jan 2000
Gao B, Flores S C, McCord J M
Abstract excerpt
The well-studied cytosolic Cu,Zn-superoxide dismutase (SOD) protects against reperfusion injury, although its short (6 min) plasma half-life and negative charge create undesirable pharmacokinetics. We have designed, cloned, and expressed a genetic variant of SOD with altered pharmacological prope...
Topics
- Amino Acid Sequence
- Arginine
- Binding Sites
- Chromatography, Affinity
- Cloning, Molecular
- Escherichia coli
- Genetic Variation
- Glycine
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Proteoglycans
- Recombinant Fusion Proteins
- Restriction Mapping
- Superoxide Dismutase