Article
The osmotic integrity of the yeast cell requires a functional PKC1 gene product.
Molecular and cellular biology - 1 Nov 1992
Paravicini G, Cooper M, Friedli L, Smith D J, Carpentier J L, Klig L S, Payton M A
Abstract excerpt
Seven temperature-sensitive cell lysis (cly) mutant strains of Saccharomyces cerevisiae were isolated which lyse at the restrictive temperature on hypotonic but not on osmotically supported medium. The seven mutants fell into four complementation groups, CLY12 to CLY15. The wild-type CLY15 gene w...
Topics
- Culture Media
- Flow Cytometry
- Gene Deletion
- Genetic Complementation Test
- Genetic Linkage
- Microscopy, Electron
- Mutation
- Protein Kinase C
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic
- Water-Electrolyte Balance