Article
Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol.
Molecular microbiology - 1 Apr 2001
Tomoyasu T, Mogk A, Langen H, Goloubinoff P, Bukau B
Abstract excerpt
We investigated the roles of chaperones and proteases in quality control of proteins in the Escherichia coli cytosol. In DeltarpoH mutants, which lack the heat shock transcription factor and therefore have low levels of all major cytosolic proteases and chaperones except GroEL and trigger factor, 5-10% and 20-30% of total protein aggregated at 30 degrees C and 42 degrees C respectively. The aggregates contained...
Topics
- Bacterial Proteins
- Cytosol
- Escherichia coli
- Escherichia coli Proteins
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Heat-Shock Response
- Molecular Chaperones
- Mutation
- Protein Denaturation
- Protein Folding
- Sigma Factor
- Temperature
- Transcription Factors