Stability of producer hybridoma cell lines after cell sorting: a case study.

Abstract excerpt

Flow cytometry was used in combination with immunofluorescent staining for intracellular and surface-associated antibody contents to identify a significant non-producer cell fraction in a murine hybridoma cell line that had shown a decline in monoclonal antibody productivity with passaging. Viabl...

Topics

• Animals
• Antibodies, Monoclonal
• Cell Cycle
• Cell Division
• Cell Separation
• Flow Cytometry
• Fluorescent Antibody Technique
• Hybridomas
• Kinetics
• Mice
• Mutation