Article
Application of population balance model to the loss of hybridoma antibody productivity.
Biotechnology progress - 1 Jan 2000
Lee G M, Varma A, Palsson B O
Abstract excerpt
A simple dynamic model has been applied to explain the population dynamics of monoclonal antibody (MAb) producing (producer) and nonproducing hybridoma cells (nonproducer) coexisting in culture. The events of mutation or loss of genes associated with antibody synthesis have been incorporated into the model to account for the conversion of a producer to a nonproducer. The model shows that the cell population is...
Topics
- Antibodies, Monoclonal
- Cell Division
- Cells, Cultured
- Hybridomas
- Kinetics
- Mathematics
- Models, Biological
- Mutation