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Optimization of Sorting Conditions for the Selection of Stable, High‐Producing Mammalian Cell Lines

1 Jan 1999

Abstract excerpt

The production of Green Fluorescent Protein in recombinant NIH3T3 mouse fibroblast cells was used as a model to determine the optimal conditions for the rapid isolation of high-producing cell lines with a fluorescence-activated cell sorter. "Bulk sorting", that is, sorting of a large number of positive cells, did not result in a stable, high-producing cell line due to overgrowth of high-producing cells by low- or...

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PMID: 10514268
DOI: 10.1021/bp990089g

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