Article
Identification of a hypothetical protein of plant pathogenic Xanthomonas campestris as a novel beta-galactosidase.
Journal of molecular microbiology and biotechnology - 1 Jan 2007
Yang Tsuey-Ching, Hu Rouh-Mei, Weng Shu-Fen, Tseng Yi-Hsiung
Abstract excerpt
Xc17L, a lactose-utilizing mutant of Xanthomonas campestris pv. campestris previously isolated by mutagenesis with nitrous acid, displays a level of beta-galactosidase 3.5-fold higher than that in the parental Xc17. In this study, the gene encoding the enzyme displaying a higher specific activity in Xc17L was inactivated by mini-Tn5 transposition. Sequencing revealed that the product (579 aa, 63.5 kDa) of this...
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