Article
Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
Journal of bacteriology - 1 May 1990
Hötte B, Rath-Arnold I, Pühler A, Simon R
Abstract excerpt
Cosmid clones able to restore exopolysaccharide production in possibly insertion sequence element-induced surface mutants of Xanthomonas campestris pv. campestris were isolated. By fragment-specific Tn5-lac mutagenesis of one of the cosmids, pXCB1002, a new DNA region which is involved in exopolysaccharide biosynthesis and which is organized into at least 12 complementation groups was identified.
Topics
- Chromosome Mapping
- Cloning, Molecular
- Cosmids
- DNA, Bacterial
- Gene Library
- Genes, Bacterial
- Genetic Complementation Test
- Phenotype
- Polysaccharides, Bacterial
- Xanthomonas